Threshold of pathological proteinuria measured with Coomassie Brilliant Blue.
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Criticism of the use of Coomassie Brilliant Blue G-250 for the quantitative determination of proteins.
The quantitative determination of proteins in biological fluids, using Coomassie Brilliant Blue G-250, was evaluated. Compared with the biuret method, the Coomassie Blue G-250 method needs a much shorter time for analysis and has a greater sensitivity. The sensitivity of the dye for albumin is significantly greater than for globulins. The standard curves for the biuret method are more linear th...
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The sorption studies of coomassie brilliant blue (CBB) from aqueous solution have been carried out on wheat bran (WB). Coomassie brilliant blue on wheat bran was used to study the adsorption behavior under various parameters such as pH, dosage amount, and contact time. It was observed that under optimized conditions up to 95.70% dye could be removed from solution onto WB. Langmuir and Freundlic...
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Blue native gel electrophoresis (BN-PAGE) is used extensively for characterization of mitochondrial respiratory complexes and uses the binding of Coomassie brilliant blue G-250 to visualize proteins. Oxidative modification of sulfhydryl groups of such proteins can be evaluated by labeling with iodoacetamide conjugated to biotin (BIAM) and detected with streptavidin peroxidase on Western blots f...
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staff Coomassie Brilliant Blau G250. J Clin Chem Cm Biochem 1981;19:203-8. 5. Peterson GL Determination of total protein.Methods Enzymol 1983;91:95-119. 6. SedmakJJ,GroesbergSE.A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250. Anal Biochem 1977;79:544-52. 7. JohnsonA, Lott JA. Standardization of the Coomassie Blue method forcerebrospinal fluid proteins. Cl...
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In this study, based on colony morphology characteristics, a total of 19 fungal endophytes were isolated from root of Calotropis Procera a traditional Indian medicinal plant. All fungal isolates were screened for their dye degradation ability. The dyes used as test dyes were Rose Bengal (RB), azo dye Methyl Red (MR), Coomassie Brilliant Blue (CBB) and Methylene Blue (MB) and the concentration o...
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عنوان ژورنال:
- Clinical chemistry
دوره 31 1 شماره
صفحات -
تاریخ انتشار 1985